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KMID : 0880220190570100910
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Journal of Microbiology
2019 Volume.57 No. 10 p.910 ~ p.917
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RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli
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Lee Jae-Jin
Lee Dong-Ho
Jeon Che-Ok
Lee Kang-Seok
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Abstract
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Studies have shown that many enzymes involved in glycolysis are upregulated in Escherichia coli endoribonuclease G (rng) null mutants. However, the molecular mechanisms underlying the RNase G-associated regulation of glycolysis have not been characterized. Here, we show that RNase G cleaves the 5′ untranslated region of triosephosphate isomerase A (tpiA) mRNA, leading to destabilization of the mRNA in E. coli. Nucleotide substitutions within the RNase G cleavage site in the genome resulted in altered tpiA mRNA stability, indicating that RNase G activity influences tpiA mRNA abundance. In addition, we observed that tpiA expression was enhanced, whereas that of RNase G was decreased, in E. coli cells grown anaerobically. Our findings suggest that RNase G negatively regulates tpiA mRNA abundance in response to oxygen availability in E. coli.
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KEYWORD
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glycolysis, mRNA abundance, rng, RNase G, tpiA
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